With the help of laboratory experiments, basic molecular techniques will be taught,
including the isolation of plasmids and total DNA as well es DNA fragments from
electrophoretic agarose gels, cloning of PCR products (enzymatic modification, ligation),
transformation and in vivo amplification of plasmids, DNA blotting, labeling of DNA
probes for hybridization with nonradioactive labels (DIG-dUTP), Southern hybridization
and detection of hybridized probes by chemiluminescence, ITS-RFLP-analysis of
pathogens of oilseed rape, real-time PCR for mycotoxin-producing pathogens of cereals.
In accompanying lectures, aspects of nucleic acid and protein chemistry relevant
for analytical techniques will be explained. Technical solutions for specific scientific
questions will be presented and discussed.
Learning outcome, core skills:
Technical skills in nucleic acid nucleic acid analysis techniques; ability to select adequat
methods for a research project. Presentation and interpretation of the results.
Examination: Oral examination (approx. 30 minutes)
Submitted and accepted protocol
Basic knowledge of the structure and properties of nucleic acids, properties and
applications of enzymes used in the analysis and manipulation of DNA, understanding
of standard techniques (Southern blotting, electrophoresis, DNA sequencing), data
analysis, criteria for the selection of a DNA analytical technique for a specific task.